Fig. 2
From: Hdac7 promotes lung tumorigenesis by inhibiting Stat3 activation
Hdac7 mutant suppresses proliferation and enhances apoptosis of lung tumor cells in mice. a. Left panels: Representative florescent images of mouse lung sections stained for EdU-positive cells (red) in lung tumors. Right panel: Statistics analysis of EdU-positive cells per field. b. Left panels: Representative florescent images of mouse lung sections for TUNEL staining of apoptotic cells (green) in lung tumors. Right panel: representative statistics analysis of TUNEL positive cells per field. The genotypes of the mice were indicated above the images. Hoechst and DAPI was used for staining for nucleus, respectively. Bar 50μm. The EdU-positive or TUNEL-positive cells in tumor area of six randomly chosen fields/lung were counted on digital fluorescence images and more than five animals for each genotype were included. Values represent the means ± SD. ***, p < 0.001. c. Western blotting for caspase-3 cleavage and phosphorylation of Histone H3 in lung tumors from Hdac7 +/−/K-Ras and & control mice