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Fig. 5 | Molecular Cancer

Fig. 5

From: Hdac7 promotes lung tumorigenesis by inhibiting Stat3 activation

Fig. 5

dnStat3 reverses Hdac7 mutant –mediated tumor inhibition in mice and partially rescues HDAC7 depletion-mediated growth suppression of human lung cancer cells. a Representative Western blot analysis of the STAT3 protein level and phosphorylation of endogenous STAT3 proteins of shHDAC7 or scramble shRNA-expressing H1299 cells infected with lenti-Cre or lenti-Cre-2A-dnStat3 viruses, respectively. b Statistical analyses of tumor numbers on lung surfaces of Hdac7 +/−/K-Ras and control mice infected with Lenti-Cre or Lenti-Cre-2A-dnStat3 viruses, respectively. Each dot/square represents an individual mouse. c Representative histologic images of H&E stained lung sections (left panels) and statistical analyses (right panel) of tumor burden (right panel) of the same mice in (b). d The growth curves of the same lung cancer cells in (a). MTT assay showed that HDAC7 knockdown-mediated cell growth suppression was partially restored by expressing dnStat3. e. Representative photographs (left panel) and statistical analysis (right panel) of the soft agar colony formation assay for the same lung cancer cells in (a). Values in (b-e) represent the means ± SD of three experiments. **, p < 0.01; ***, p < 0.001; ns, not significant

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