Fig. 7

TDGF-1 endogenous expression and effect on proliferation, migration, invasion and apoptosis of GSCs. a TDGF-1 protein expression levels in normal brain tissues (NBTs), low-grade glioma tissues (WHO I-II) and high-grade glioma tissues (WHO III-IV) are shown. Data are presented as the mean ± SD (n = 3,each group). b The expression of TDGF-1 in human astrocytes (HA) and glioblastoma cell lines (U87 and U251). c The expression of TDGF-1 in glioblastoma cell lines (U87 and U251) and glioblastoma stem cells (GSC-U87, GSC-U251). d CCK-8 assay was used to measure the effect of TDGF-1 on the proliferation of GSC-U87 and GSC-U251 cells. e The apoptotic percentages of GSC-U87 and GSC-U251 were detected after TDGF-1 over-expression or knockdown. f Transwell assays were used to measure the effect of TDGF-1 on cell migration and invasion of GSC-U87 and GSC-U251 cells. Data are presented as the mean ± SD (n = 5, each group). ^** P < 0.01 vs. TDGF-1(+)NC, ^## P < 0.01 vs. TDGF-1(−)NC. g Western blot assay of the p-JAK-1/JAK-1 and p-STAT3/STAT3 expression regulated by TDGF-1. Data are presented as the mean ± SD (n = 5, each group). ^** P < 0.01 vs. TDGF-1(+)NC, ^## P < 0.01 vs. TDGF-1(−)NC. h Weatern blot assay were used to detect the TDGF-1 expression regulated by miR-194-5p and SOX3. Data are presented as the mean ± SD (n = 5, each group). ^** P < 0.01 vs. AgomiR-194-5p-NC + SOX3(+)NC group, ^## P < 0.01 vs. AgomiR-194-5p + SOX3(+)NC group. i Weatern blot assay were used to detect the TDGF-1 expression regulated by miR-122 and SOX3. Data are presented as the mean ± SD (n = 5, each group). ^** P < 0.01 vs. AgomiR-122-NC + SOX3(+)NC group, ^## P < 0.01 vs. AgomiR-122 + SOX3(+)NC group