Fig. 5From: Long noncoding RNA NORAD, a novel competing endogenous RNA, enhances the hypoxia-induced epithelial-mesenchymal transition to promote metastasis in pancreatic cancerNORAD promotes EMT via regulating RhoA expression. a Western blot analysis of E-cadherin, N-cadherin, Vimentin and ZEB1. GAPDH was used as a loading control. Relative density was shown below each lane; b Western blot analysis of E-cadherin, RhoA and RhoA downstream protein Rock1. Relative density was shown below each lane; c transwell migration and invasion assays, NC: negative control, NORAD: cell lines with NORAD overexpressed, NORAD + CCG-1423: NORAD overexpressed cell lines incubated with 300 nM CCG-1423 72 h; d Histograms of numbers of migration and invasion cells*, statistical significance, P < 0.05, **p < 0.01, ***p < 0.001Back to article page