Fig. 3
From: Bi-directional exosome-driven intercommunication between the hepatic niche and cancer cells
Exosomes are produced by the liver MPS. a Transmission electron microscopy (TEM) of concentrated effluent from the liver MPS with or without 500 MDA-231 cells within the 1.2 million liver cells. HepN = Hepatic Niche (includes hepatocytes and non-parenchymal cells). b Exosomes from the MPS with breast cancer cells have extracellular vesicles of varied sizes. Left: representative TEM, Right: quantitation of exosome size (maximum diameter in nm). c Exosome protein arrays performed on exosome protein extracts from HepN and HepN + MDA-231 in the Liver MPS and MDA-231 in 2D cultures, with (d) relative quantification. e Analysis of miRNA content of the exosomes isolated from the liver MPS. Shown are representative examples of experiments performed at least 3 times