Table 1 Techniques used for chromosome conformation capture

Data

One-one

One-all

Many-many

All-all

Many-many

Readout

PCR

Inverse PCR, microarrays, NGS

LMA, sequencing

Sequencing

Sequencing

Advantages

Simple, cheap

Relatively simple, goal-oriented

Explores interactions among many fragments, reduces bias, relatively targeted

Genome-wide interactions

Protein bonded interactions

Limitations

Laborious, only known combinations, poor resolution

Only chosen regions

Difficult to design hundreds of primers, analyzes only chosen fragments, some long-distance interactions are missing

Difficult to sequence and analyze, expensive

Analyzes only chosen protein

Ref.

[3, 10]

[12]

[17, 18, 142]

[19]

[24, 25]