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Fig. 3 | Molecular Cancer

Fig. 3

From: Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer

Fig. 3

MALAT1 promotes autophagy in GC cells. a Relative mRNA levels of specific lncRNAs in SGC7901/VCR and SGC7901 cells using real-time PCR. b SGC7901/VCR cells stably transfected with shRNA-MALAT1 or a control were subjected to Western blot analysis of LC3-II and p62. c Autophagy was evaluated using transmission electron microscopy in SGC7901/VCR stably transfected with shRNA-MALAT1 or a control. d Silencing of MALAT1 sensitized SGC7901/VCR cells to chemotherapeutic agents as evidenced by decreased IC50 values. e SGC7901/VCR cells were treated with the cisplatin (10 μg/ml) for 24 h. Total protein as well as cytoplasmic protein fractions were isolated, and the indicated proteins were detected by Western blot. The data are presented as the means ± S.D. of values obtained in 3 independent experiments. *, p < 0.05

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