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Fig. 4 | Molecular Cancer

Fig. 4

From: Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer

Fig. 4

MALAT1 regulates ATG12. a The mRNA expression of the indicated autophagy related genes was measured using real-time PCR in SGC7901/VCR cells stably transfected with shRNA-MALAT1 or a control. Student t tests were used to determine the statistical significance of the differences between the groups. b Western blot analysis of ATG12 and ATG3 was performed in SGC7901/VCR cells stably transfected with shRNA-MALAT1 or a control. c The mRNA or protein levels of ATG12 were determined using real-time PCR and Western blot analysis in SGC7901/VCR cells stably transfected with shRNA-MALAT1 or a control. d Biotinylated MALAT1 or antisense RNA was incubated with cell extracts of SGC7901/VCR cells, targeted with streptavidin beads, and washed, and the associated proteins were resolved on a gel. Western blot analysis detected the specific association of EZH2 and MALAT1 (n=3). e EZH2 knockdown efficiency was confirmed by Western blot. qRT-PCR analysis of putative PRC2 target genes after MALAT1 and EZH2 knockdown, respectively. f ChIP analysis of H3K27 trimethylation status of candidate EZH2 target genes after knockdown assay. The data are presented as the means ± S.D. of values obtained in 3 independent experiments. #, p < 0.05. *, p < 0.05. n.s., not significant

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