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Fig. 6 | Molecular Cancer

Fig. 6

From: Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer

Fig. 6

MALAT1 relieves the inhibitory effect of miR-23b-3p on ATG12. a SGC7901/VCR cells were infected with lentiviruses expressing control shRNA or MALAT1 shRNA. Forty-eight hours after infection, total RNA was subjected to real-time RT–PCR analysis. b The protein levels of ATG12 in SGC7901/VCR cells transfected with mimic control, miR-23b-3p mimics, miR-23b-3p mimics +LV-MALAT1. c The protein levels of ATG12 in SGC7901/VCR cells transfected with inhibitor control, miR-23b-3p inhibitor, miR-23b-3p inhibitor+shRNA-MALAT1. d Overexpression of miR-23b-3p binding-defective MALAT1 had no significant effect on the expression of ATG12 in SGC7901/VCR cells. e, f Luciferase activity in SGC7901/VCR cells transfected with luciferase reporters containing ATG12 3’-UTR or nothing. Data are represented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. Error bars represent the mean±S.D. of triplicate experiments. *, p < 0.05

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