Fig. 3

JMJD6 Promotes Melanogenesis in Melanoma Cells. a A375 and 451Lu cells were transfected with control siRNA/vector, siJMJD6#1, siJMJD6#2, JMJD6, or JMJD6m. Total RNAs were extracted and analyzed for the mRNA expression of MITF by qRT-PCR. Results were presented as the mean ± SD. b A375 and 451Lu cells were transfected with control siRNA/vector, siJMJD6#1, siJMJD6#2, JMJD6, or JMJD6m. Western blotting analysis was performed with antibodies against indicated proteins. c B16F10 cells were infected with lentiviruses carrying control shRNA or Jmjd6 shRNA, and/or infected with retroviruses carrying vector or expression plasmids for PAK1, PAK1Δ15, JMJD6, or JMJD6m, and treated with α-MSH (100 nM). The culture dishes of B16F10 cells were photographed. d The B16F10 cells infected with viruses carrying indicated constructs were incubated with α-MSH (100 nM) for 24 h. Melanin content was measured at 475 nm and normalized to the protein concentration. Each bar represents the mean ± SD. Each independent experiment was performed at least three times