Fig. 5

JMJD6 Enhances the Angiogenic Potential of Melanoma Cells. a A375 cells and HUVECs were infected with lentiviruses carrying control shRNA, JMJD6 shRNA or PAK1 shRNA, and/or infected with retroviruses carrying PAK1, PAK1Δ15, JMJD6, or JMJD6m. Then, the cells were added onto a solidified extracellular matrix and were treated with or without 20 ng/ml VEGF. After incubation, the number of endothelial tubes was counted under a light microscope. Representative images from each group were shown. Each bar represents the mean ± SD. Each independent experiment was performed at least three times. b A375 cells were infected with viruses carrying indicated constructs for YSM analysis. The number of blood vessels that migrated into the sponge was counted. Representative images from each group are shown. Each bar represents the mean ± SD. Scale bar = 250 μm. Each independent experiment was performed at least three times. c Matrigel only or Matrigel mixed with A375 cells infected with viruses carrying control shRNA or JMJD6 shRNAs were subcutaneously injected into 6-week-old BALB/c female mice (n = 6). The Matrigel plugs were removed for H&E and Masson trichrome staining. Scale bar = 200 μm. The relative photos are shown. Results were presented as the mean ± SD