Fig. 2
From: Generation of stable PDX derived cell lines using conditional reprogramming
CR-PDX cell lines are amenable to in vitro preclinical applications. a CR-PDX cells were removed from CR conditions and treated with the indicated inhibitors (n = 3, mean ± SD shown). In vitro drug sensitivity of CR-PDX cells was compared to in vivo response of the parental tumor. Means ± error are shown for each model. N = 8 (OV0857F), n = 8 (HLXF-036LN), n = 9 (LG0567F), and n = 10 (HLXF-056) (b) CR-PDX cells are amenable to gene knockdown studies using siRNA. CR-HLXF-036LN cells were reverse transfected with indicated siRNAs for 72 h. Knockdown efficiency was confirmed by qPCR and the effect of knockdown on viability was assessed at the end of study, (N = 3, mean ± SD shown, ***p < 0.05, Student’s t-test, two-tailed)