Fig. 2

miR-124 reinforcement inhibits survival of breast cancer cells in bone microenvironment. a Images of luciferase signal in tibias of nude mice transplanted with luciferase-labeled MDA-MB-231 cells overexpressing miR-124 or negative control (NC). b miR-124 overexpression reduced the quantification of bone lesions detected by bioluminescence. Horizontal line indicates median value. P = 0.0003 by Mann–Whitney U test. c Effects of miR-124 on breast cancer-induced osteolytic lesions in mice as shown by X-ray imaging. Arrows indicate osteolytic lesions (Top). Statistical analysis showed reduced osteolytic lesion area in miR-124 group (Bottom). d Effects of miR-124 on the number and activity of osteoclasts in the tumor-bone interface as detected by TRAP staining (red signal). Mouse tibias were collected from each group and sectioned for H&E staining (left) at an original magnification of ×100 and TRAP staining (right) at an original magnification of ×100 and ×200