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Fig. 1 | Molecular Cancer

Fig. 1

From: HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma

Fig. 1

HSP90 bound to PKM2 and increased the abundance of PKM2 protein in HCC cells. a Flag-HSP90 and HA-PKM2 plasmids were transfected into HEK293 cells. Immunoprecipitation was performed using anti-Flag or anti-HA antibody. HSP90 and PKM2 showed an interaction between each other in HEK293 cells. b The interaction between endogenous HSP90 and PKM2 proteins in Hep3B cells was analyzed by Co-IP. Endogenous HSP90 and PKM2 proteins interacted with each other in Hep3B cells. c GST pull-down assays were performed to investigate the direct interaction between HSP90 and PKM2. d Hep3B cells were transfected with HSP90 shRNA or negative-control (NC) shRNA. 72 h after transfection, the levels of HSP90 and PKM2 protein in Hep3B cells were examined. Knockdown of HSP90 decreased PKM2 protein in Hep3B cells. e Huh7 cells were transduced with retroviruses encoding Flag-HSP90 or empty control retroviruses. 72 h after viral transduction, the levels of HSP90 and PKM2 protein in Huh7 cells were examined. Forced expression of HSP90 increased PKM2 protein abundance in Huh7 cells. *, P < 0.05 by t-test

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Molecular Cancer

ISSN: 1476-4598