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Fig. 3 | Molecular Cancer

Fig. 3

From: HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma

Fig. 3

HSP90 regulated the stability of the PKM2 protein in HCC cells. a Huh7 cells were transfected with empty vector or Flag-HSP90 vector. Hep3B cells were transfected with negative control (NC) shRNA or HSP90 shRNA. qRT-PCR demonstrated that neither HSP90 overexpression or knockdown changed the mRNA level of PKM2. b & c The protein half-life of PKM2 in Hep3B cells was analyzed following treatment with cycloheximide (CHX). The PKM2 turnover rate was lower in Hep3B cells with HSP90 knockdown and it was higher in HSP90 overexpressing Huh7 cells. d MG-132 was used to inhibit the proteasomal degradation in Hep3B cells. MG-132 treatment reversed the downregulation of PKM2 protein induced by HSP90 knockdown. *, P < 0.05 by one way ANOVA test

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