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Fig. 5 | Molecular Cancer

Fig. 5

From: HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma

Fig. 5

HSP90 increases PKM2 phosphorylation through GSK-3β. a Co-IP was performed for HSP90, PKM2 and GSK-3β to confirm whether these protein formed protein complex in Huh7 cells. b Huh7 cells transfected with HSP90 vector or control vector were treated with GSK3i IX, a GSK-3β inhibitor. Phosphorylation of PKM2 was examined by western blot after inhibiting GSK-3β activity. c Huh7 cells transfected with HSP90 vector or control vector were treated with GSK-3β siRNA to knockdown GSK-3β. Phosphorylation of PKM2 was examined by western blot after GSK-3β knockdown. d In vitro kinase assay to determine the effects of recombinant GSK3β on threonine phosphorylation of PKM2-WT or T328A

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Molecular Cancer

ISSN: 1476-4598