Fig. 3
From: Impact of FHIT loss on the translation of cancer-associated mRNAs
Fhit-mediated changes in target protein expression are independent of mRNA level. a D1 and E1 cells treated for 24 h with Ponasterone A were analyzed for changes in TP53I3, IFIT1, EEF2 and Vinculin. Shown are representative Western blots for each of triplicate determinations and the fold change normalized to Vinculin is presented beneath the panels for TP53I3, IFIT1 and EEF2. b Cytoplasmic extracts from these cells were spiked with luciferase RNA as a control for sample recovery and analyzed by RT-qPCR for changes in steady-state levels of TP53I3, EEF2, IFIT1 and Vinculin mRNA. The mean fold change ± SEM (n = 3) determined for each mRNA by by RT-qPCR (black bars) is shown alongside the average difference from the duplicate RNA-Seq datasets. c The analysis in a was repeated for ADAM9 and FASN, with U2AF35 used as a normalization control