Fig. 3
From: The Jun/miR-22/HuR regulatory axis contributes to tumourigenesis in colorectal cancer
miR-22 and miR-129 can inhibit HuR by binding to its 3’-UTR. a Western blot analysis of HuR levels in normal colon mucosal epithelial cell line NCM460 and 7 CRC cell lines. b-e qRT-PCR analysis of miR-22 and miR-129 levels and the correlation between miRNA and HuR levels in the aforementioned 8 cell lines. f-i qRT-PCR analysis of miR-22 and miR-129 levels and the correlation between fold changes of miRNA and HuR levels in CRC tissue pairs. j Western blot analysis of HuR levels in 3 CRC cell lines after treatment with miR-22/miR-129 mimic or inhibitor. k Relative luciferase activities in SW480 treated with a miR-22/miR-129 mimic or inhibitor. **P < 0.01; ***P < 0.001