Fig. 2

miR-494 promotes the proliferation of CRC cells. a The effect of miR-494 overexpression on cell cycle progression. HCT-116 cells were transfected with a control miRNA or a miR-494 mimic for 24 h and then switched to conditioned medium without serum for 24 h. The cells were cultured in medium containing 10% FBS for 24 h and collected for cell cycle analysis by flow cytometry. The data shown in the right-hand panel are representative of three independent experiments. Representative profiles are shown in the left-hand panel. b The effect of miR-494 knockdown on cell cycle progression. After HCT-116 cells were transfected with the control miRNA inhibitor or the miR-494 inhibitor for 24 h, cells were switched to conditioned medium without serum for 24 h. The cells were then cultured in medium containing 10% FBS for 24 h and collected for cell cycle analysis by flow cytometry. The data shown in the right-hand panel are representative of three independent experiments. Representative profiles are shown in the left-hand panel. c Colony formation assay. HCT-116 cells stably expressing the indicated miRNAs were maintained in culture media for 7 days and stained with crystal violet. The number of colonies in each condition was counted, and is expressed as a mean ± S.D. from triplicate experiments. *, P < 0.05 (Student’s t-test). d The effect of miR-494 on cell proliferation. HCT-116 cells were transfected with the indicated constructs, and cell proliferation was measured by BrdU incorporation. Data are mean ± S.D. from triplicate experiments. *, P < 0.05 (Student’s t-test). e The effect of miR-494 on cell proliferation. HCT-116 cells were transfected with a miR-494 mimic or with a miR-494 inhibitor. CCK8 assays were performed at 1, 2, 3, and 4 days post-transfection. Data are mean ± S.D. from triplicate experiments. **, P < 0.01;***, P < 0.001, compared to Anti-Control. #, P < 0.05; ##, P < 0.01; ###, P < 0.001, compared to miR-Control, Student’s t-test