Fig. 6
From: MicroRNA-494 promotes cancer progression and targets adenomatous polyposis coli in colorectal cancer
APC is functionally involved in miR-494–induced β-catenin signaling activity of CRC cells. a Dual luciferase experiment demonstrating the effects on Top/Fop reporter activity in the indicated cells. Values were normalized to a renilla transfection control. The figure shown is representative of at least three independent experiments. b Dual luciferase assay showing the effects on a c-Myc reporter construct in the indicated cells. c Dual luciferase assay showing the effects on a cyclin D1 reporter construct in the indicated cells. d Dual luciferase experiment demonstrating the effects on Top/Fop reporter activity in the indicated cells following miR-494 downregulation. e Dual luciferase assay showing the effects on a c-Myc reporter construct in the indicated cells following miR-494 downregulation. f Dual luciferase assay showing the effects on a cyclin D1 reporter construct in the indicated cells following miR-494 downregulation. g APC western blot of the indicated cells. (**, P < 0.01, Student’s t-test, n = 3 independent experiments)