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Fig. 4 | Molecular Cancer

Fig. 4

From: STAT3-induced lncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy

Fig. 4

STAT3 induces HAGLROS expression as a transcription factor. a The predicted sites of STAT3-binding in human HAGLROS promoter by gene sequence analysis. b The effect of STAT3 knockdown on HAGLROS expression and the transfection efficiency of STAT3 siRNAs. c The effect of STAT3 overexpression on HAGLROS expression and the transfection efficiency of STAT3 plasmid. d Quantitative ChIP assays were used to show direct binding of STAT3 to endogenous HAGLROS promoter regions. e The representative blot of the binding of STAT3 to endogenous HAGLROS promoter regions. f and g A dual-luciferase reporter assay was performed by co-transfecting the full-length HAGLROS promoter (HAGLROS-F) or deleted HAGLROS E1 or E2 fragment (HAGLROS-P2 and HAGLROS-P1) with STAT3 plasmid or empty vectors in 293T cells. Error bars indicate the means ± S.E.M. *P < 0.05, **P < 0.01, # P > 0.05

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