Fig. 5

Expression of PAI-2 in CCA tissues. a Real-time PCR of miR-15a in 14 CCA tissues and 2 normal liver tissues. Bars represent mean ± SD of three measurements. Dashed line represents the level of average expression in normal liver tissues. b Real-time PCR of PAI-2 in 14 CCA tissues and 2 normal liver tissues. Bars represent mean ± SD of three measurements. c Immunohistochemical staining of PAI-2 in clinical CCA samples. Representative images of high and low expression of PAI-2 are shown. Scale bar: 200 μm. d The survival analysis by Kaplan-Meier test was performed using 3-y as the cut-off. e The proposed mechanism of miR-15a-PAI-2 axis in microenvironment of CCA tissue