|
qPCR-Primer
|
|
XLOC_000647
|
+
|
5’-GCATCCACGCCTGGTGACAAC-3′
|
|
-
|
-5’-GTGACTCCGACAGCCCTTGCC-3’
|
|
NLRP3
|
+
|
5’-CTTGCATCAGTATTGAGCACCA-3′
|
|
-
|
5’-CCAGTTTCTGCAGGTTACACT-3’
|
|
ACTB
|
+
|
5’-CCAACCGCGAGAAGATGACC-3′
|
|
-
|
5’-AGTCCATCACGATGCCAGT-3’
|
|
shRNA
|
|
shNLRP3
|
+
|
5’-GGATCTTCGCTGCGATCAACATTCAAGAGATGTTGATCGCAGCGAAGATCCTTTTTTA-3′
|
|
-
|
5’-TAAAAAAGGATCTTCGCTGCGATCAACATCTCTTGAATGTTGATCGCAGCGAAGATCCGGA-3’
|
|
pSH-U6
|
+
|
5’-GCACCCAGTCCGCCCTGAGCAAATTCAAGAGATTTGCTCAGGGCGGACTGGGTGCTTTTT-3′
|
|
-
|
5’-AAAAAGCACCCAGTCCGCCCTGAGCAAATCTCTTGAATTTGCTCAGGGCGGACTGGGTGC-3’
|
