Fig. 4

Nischarin Decreases Invadopodia Formation. a MDA-MB-231 (n = 70) and MDA-MB-231 Nisch cells (n = 20) were seeded on a fluorescent gelatin matrix (green) and allowed to degrade for 4 h. b 231 (n = 61) and 231 Nisch cells (n = 27) were seeded on a fluorescent gelatin matrix (green) and allowed to degrade for 5 h. Cells were then fixed and stained with Phalloidin (red) to visualize the cells degrading using the Nikon Eclipse Ti. The percentage of cells degrading was determined manually. All experiments were performed in triplicates. c WT and Null MEFs were seeded on decellularized NIH3T3 FDMs for 24 h. Cells were then fixed, stained with Fibronectin (green) and Phalloidin (red), then visualized using the Nikon Eclipse Ti. Mean fluorescence was determined by ImageJ. d Fibroblast-Derived Matrices (FDMs) were generated from WT (n = 5) and HET (n = 5) MEFs and fiber number (bottom left) and width (bottom right) were quantitated by CT-FIRE. e MCF7 and f MCF10A cells were seeded in an invasion chamber with either serum-free media or media from cultured 231 or 231 Nisch cells. 24 h later, invaded cells were manually counted after crystal violet staining. g Fluorescent gelatin matrix degradation of MCF7 cells cultured in media isolated from cultured 231 or 231 Nisch cells. The percentage of cells degraded was determined manually