From: Nuclear functions of mammalian MicroRNAs in gene regulation, immunity and cancer
Cell line | Method | Result | Year |
---|---|---|---|
L6 rat myoblast (nucleolus) | Microarray | One third of the detected miRNAs exhibited nucleolar expression at least as high as those observed in the cytoplasm | 2009 [26] |
In situ hybridization | MiR-351, miR-494, miR-664, miR-1, miR-206 are concentrated in the nucleolus MiR-199a-3p, miR-21, miR-125a-5p, and let-7a are more concentrated in the nucleoplasm and cytoplasm | ||
RT-qPCR | MiR-351, miR-1, miR-206, miR-21Â are concentrated in the nucleolus MiR-494, miR-664Â are more concentrated in the nucleoplasm and cytoplasm | ||
Human nasopharyngeal carcinoma (NPC) 5-8F cell line | Deep sequencing | Among 339 nuclear and 324 cytoplasmic miRNAs, 300 of them overlap | 2010 [27] |
HCT116 human colorectal carcinoma cell | Microarray | The overall average of nuclear ratio of miRNAs is 0.471 ± 0.15 | 2010 [28] |
RT-PCR | MiR-16, miR-19b, miR-200b, miR-222, miR-29b, miR-29c are highly expressed in the nucleus | ||
Northern blot | MiR-19b, miR-195Â are highly expressed in the nucleus | ||
Human monocytic leukemia cell line (THP-1) | Deep sequencing | MiR-16, miR-15b and miR-374b are all over 2-fold enriched in the nucleus; Nuclear/cytoplasmic ratios of 16 microRNAs are over 1. | 2010 [134] |
Northern blot | MiR-15 (both a and b) and miR-16 are over 2-fold enriched in the nucleus | ||
C57BL/6 J mouse liver cell | Microarray | MiR-709, miR-805, miR-690 and miR-122 are enriched in the nucleus | 2012 [49] |
RT-qPCR | MiR-709, miR-690 and miR-30e are highly expressed in the nucleus | ||
The human breast cancer cell MDA-MB-231 | High-throughput sequencing | Almost one-fifth of nuclear small RNAs are annotated as piRNAs | 2012 [31] |
The human breast cancer cell line MCF-7(noninvasive breast cancer cells), MDA-MB-231(invasive breast cancer cell) and the human mammary epithelial cell line MCF-10A (normal breast cells) | Microarray | Nuclear/cytoplasmic ratios of numerous miRNAs vary considerably across different cell lines | |
Rat primary cortical neuron | Microarray | 87 (32.6%) miRNAs are dominant in the cytoplasm, while only (1.5%) miR-133b*, miR-365*, miR-328a* and miR-92a are in the nucleus | 2013 [29] |
Deep sequencing | MiR-143 and miR-126* are enriched in the nucleus | ||
Northern blot | MiR-25, miR-92a, miR-27a, miR-92b are highly expressed in the nucleus MiR-138, miR-19b are highly expressed in the cytoplasm | ||
RT-qPCR | MiR-25 and miR-92a are highly expressed in the nucleus | ||
Fluorescence in situ hybridization | MiR-25, miR-92Â are highly expressed in the nucleus MiR-9Â is highly expressed in the cytoplasm | ||
HeLa cell (nucleolus) | RT-qPCR array | 11 miRNAs are highly expressed in the nucleolus | 2013 [30] |
HeLa cell, lung cancer cell H1299, liver cancer cell Huh7, RPE cells, human adult fibroblast AG06858 and primary mouse adult fibroblast (nucleolus) | In situ Hybridization | MiR191, miR-484, miR-574-3p and miR-193b are highly expressed in the nucleolus | |
Four murine myeloid cell lines (LSK, promyelocyte, myelocyte and granulocyte) | RT-qPCR array | Nuclear/cytoplasmic ratios of miR-706, miR-467a*, miR-709, miR-690, miR-135a* (now miR-135a-1-3p) and miR-142-3p are over 0.1 in one or more cell lines; Nuclear/cytoplasmic ratios of miR-706 and miR-467a* (now miR-467a-3p) are over 1 in promyelocytes; | 2014 [32] |
Individual RT-qPCR | MiR-706, miR-709 and miR-690 are enriched in the nucleus; MiR-467a* is enriched only in the nucleus of LSK and promyelocyte |