Fig. 2

Over-expression of cir-ITCH inhibits bladder cancer cell growth, colony formation, migration and invasion in vitro. a and b Cir-ITCH inhibited cell proliferation as indicated by CCk-8 assays in EJ and T24 cells. Data are mean ± S.D. from triplicate experiments (*P < 0.05, **P < 0.01, Student’s t-test). c and d Transwell invasion assay was measured and the results were expressed as the number of invaded cells per field compared with respective control (magnification, × 100, *P < 0.05, Student’s t-test). e and f Wound healing assay showed that cir-ITCH resulted in a slower closing of scratch wounds (*P < 0.05, **P < 0.01, Student’s t-test). g and h Cir-ITCH inhibited the colony formation of EJ and T24 cells as demonstrated by colony formation assays (*P < 0.05, **P < 0.01, Student’s t-test)