Fig. 4

Cir-ITCH acted as a sponge for miR-17 and miR-224. a Cir-ITCH in BCa cell lysis was pulled down and enriched with cir-ITCH specific probe and then detected by qRT-PCR(**P < 0.01, Student’s t-test). b miR-17 was pulled down and enriched with cir-ITCH specific probe and then detected by qRT-PCR(**P < 0.01, Student’s t-test). c miR-224 was pulled down and enriched with cir-ITCH specific probe and then detected by qRT-PCR(**P < 0.01, Student’s t-test). d and f Biotin-coupled miR-17/miR-224 captured a fold change of cir-ITCH in the complex as compared with biotin-coupled NC in biotin-coupled miRNA capture(*P < 0.05, **P < 0.01, Student’s t-test). e and g The product of d and f was detected using qRT-PCR, followed by agarose gel electrophoresis. h and i RNA FISH for cir-ITCH and miR-17/miR-224 was detected in BCa cells, miR-17 and miR-224 were co-localized with cir-ITCH in cytoplasm (magnification, × 400). Nuclei was stained blue (DAPI), cir-ITCH was stained red, miR-17/miR-224 was stained green