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Fig. 6 | Molecular Cancer

Fig. 6

From: Circular RNA circ-ITCH inhibits bladder cancer progression by sponging miR-17/miR-224 and regulating p21, PTEN expression

Fig. 6

Cir-ITCH modulated the expression of endogenous miR-17 and miR-224 targets p21 and PTEN. a Putative miR-17/miR-224 binding sequence in the 3′-UTR of p21 and PTEN mRNA. b and c cir-ITCH up-regulated the mRNA expression level of p21 and PTEN in BCa cells by qRT-PCR (*P < 0.05, **P < 0.01, Student’s t-test). c and d Circ-ITCH up-regulated the protein expression level of p21 and PTEN by western blot. e and f miR-17 and miR-224 decreased the protein expression level of p21 and PTEN, individually. g and h Dual luciferase reporter assays demonstrated that p21 and PTEN are direct target of miR-17(*P < 0.05, **P < 0.01, Student’s t-test). i and j Dual luciferase reporter assays demonstrated that p21 and PTEN are direct target of miR-224(*P < 0.05, Student’s t-test). k and l Western blot showed that mir-17 and miR-224 could partly decrease the protein expression level of p21 and PTEN which were promoted by cir-ITCH. m Mode pattern of the cir-ITCH-miR-17/miR-224-p21/PTEN regulatory network

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