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Fig. 5 | Molecular Cancer

Fig. 5

From: Wnt-signaling enhances neural crest migration of melanoma cells and induces an invasive phenotype

Fig. 5

Gene expression during neural crest induction in the zebrafish embryo and upon Wnt stimulation in SKMEL28 melanoma cells, and clinical significance. a Heatmaps of real-time qPCR gene expression analysis of the melanoma cell lines SKMEL19, 451 LU, BLM and A375 for AXIN2. TYR, MITF and the four genes INHBA, CYR61, ANGTPL4 and FABP7. Log2 transformed x-fold expression values were used for color-coding. Yellow: upregulated gene expression; blue: downregulated gene expression at 24 h treatment of the melanoma cells; black: not detectable b Western blot analysis to detect protein levels of beta-catenin, PTEN, phospo-Ser473 (AKT) and AKT in the melanoma cell lines A375, SKMEL19, BLM and 451 LU after treatment with 3T3-CM, 3T3-Wnt3a, or 0.5 μM PKF115–584 for 24 h. Beta-actin was used as loading control. Small-hairpin knockdown cells served as samples to investigate the dependence on beta-catenin. c Luciferase reporter assay (Super8xTOPFlash) indicates a 3-fold activation of the canonical Wnt−/β-catenin signaling pathway after stimulation of SKMEL28 cells with Wnt3a-conditioned medium (3T3-Wnt3a). PKF115–584 treatment (0.5 μM for 12 h) significantly inhibited reporter activity. **: p < 0.01, ***: p < 0.001, One way ANOVA

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