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Fig. 5 | Molecular Cancer

Fig. 5

From: CCL2/CCL5 secreted by the stroma induce IL-6/PYK2 dependent chemoresistance in ovarian cancer

Fig. 5

a. Real-time qPCR were performed on MSC before or after the experiment described in Fig. 1d. Relative transcript levels are represented as the log10 of ratios between the MSC after and before experiment of their 2–ΔΔCp real-time PCR values. b. OCCs (APOCC, Ovcar3 or Skov3) were treated or not with MCP-1 (10 nM), CCL5 (100 ng/ml), CXCL12 (100 ng/ml), bFGF (10 ng/ml) for 48 h prior treatment with Carboplatin (100 μM) and Taxol (0.1 μM) for 24 h. Histograms represent the percentage of living cells compared to the untreated cells. The results are presented as the mean (±SEM) of 3 different experiments. c. Western blot for IL-6 was performed on OCCs (APOCC, A; Ovcar3, O; or Skov3, S) treated or not with MCP-1 (10 nM) and CCL5 (100 ng/ml) for 48 h. d. Ovcar3, APOCC and Skov3 were treated or not with MCP-1 (10 nM) and CCL5 (100 ng/ml) for 48 h. The supernatants were harvested and an Elisa assay for IL-6 was performed on the supernatant of the cells. Histograms represent the mean (±SEM) for triplicates. p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***)

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