Fig. 1
From: Transcriptional profiles of different states of cancer stem cells in triple-negative breast cancer
Isolation and characterization of the four cell populations from PDXs. (a) The flow charts of ALDH, CD24 and CD44 for PDX1 and PDX2 by fluorescent activated cell sorting. We isolated four groups based on biomarker combinations of ALDH and CD24/CD44. (b) The limiting dilutions of cells obtained from PDX2 (VARI068) were implanted in the fourth fat pads of NOD-SCID mice. The tumor growth for each group was monitored and calculated weekly, and the CSC frequency for the group A, B, C, D was calculated based on the website http://bioinf.wehi.edu.au/software/elda/. (c) The expression of BCSC biomarkers ALDH, CD24 and CD44 in each sorted group. We compared each group with the following criteria: 1) CD24: A < B, C < D; 2) CD44: A > B, C > D; 3) ALDH: A/B > C/D. PDXs have different expressions of ALDH isoforms. P1: PDX1; P2: PDX2