Fig. 1

HNF1α upregulates the expression of HNF1A-AS1. a Experimental design for detecting HNF1α-regulated genes. Huh-7 cells infected with Lenti-HNF1α and Lenti-Control or Lenti-shHNF1α and Lenti-shNC. Total RNA was extracted from cells and subjected to microarray analysis 5 days after infection. b A heatmap of 51 annotated lincRNAs upregulated by HNF1α in Huh-7 cells. Colours represent the higher (red) or lower (blue) expression of transcripts, the global median scaled to 2-fold activation or repression, respectively (P ≤ 0.05). Black arrow denoted HNF1A-AS1. c Expression levels of the six selected lncRNAs were examined using real-time PCR in Huh-7 cells infected with lentivirus encoding HNF1α (left) or shHNF1α (right). d Expression levels of HNF1A-AS1 in different HCC cells. e Pearson’s correlation analysis showed positive correlation between the expression levels of HNF1A-AS1 and HNF1α in HCC tissues (r = 0.7599, P ≤ 0.0001, n = 75)