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Fig. 2 | Molecular Cancer

Fig. 2

From: The HNF1α-regulated lncRNA HNF1A-AS1 reverses the malignancy of hepatocellular carcinoma by enhancing the phosphatase activity of SHP-1

Fig. 2

HNF1α activates the transcription of HNF1A-AS1 by directly binding to its promoter. a Northern blotting was performed to verify the expression of HNF1A-AS1 in MHCC-L and HepG2 cells. b Sequence of full-length human HNF1A-AS1 in the liver. Nucleotides identified by RACE are indicated in red. c Schematic representation of the full-length HNF1A-AS1 gene, the predicted HNF1α-RE in the promoter region, the mutated sequence of HNF1α-RE and the fragment for the reporter plasmid. d ChIP assays revealed the binding of HNF1α to the promoter region of HNF1A-AS1. Real-time PCR (upper panel) was performed to examine DNA fragments immunoprecipitated by anti-HNF1α antibody in Huh-7 cells. Western blotting (lower panel) was used to verify the immunoprecipitation of the HNF1α protein. Human OCT1 promoter containing a consensus HNF1α response element was used as the positive control. e Luciferase reporter assays performed with a reporter plasmid carrying the HNF1α-RE in Huh-7 cells co-transfected with Flag-CMV or Flag-HNF1α. Mutation of HNF1α-RE abolished the transcriptional activity of HNF1α. Data represent the mean ± SD. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001

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