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Fig. 7 | Molecular Cancer

Fig. 7

From: The HNF1α-regulated lncRNA HNF1A-AS1 reverses the malignancy of hepatocellular carcinoma by enhancing the phosphatase activity of SHP-1

Fig. 7

HNF1A-AS1 increases the phosphatase activity of SHP-1 by interacting with the C-terminal of SHP-1. a Overexpression of HNF1α increased the phosphatase activity of SHP-1 in Huh-7 cells. The amount of SHP-1 protein used for the phosphatase assay were detected by western blotting (lower panel). b HNF1A-AS1 enhanced the phosphatase activity of SHP-1 in Huh-7 cells. c Forced expression of HNF1α induced phosphatase activity of SHP-1 activation, while knockdown of HNF1A-AS1 blocked the effect of HNF1α on SHP-1. d HNF1A-AS1 increased the phosphatase activity of the recombinant SHP-1 protein in vitro. C-terminal deletion of SHP-1 abolished the effect of HNF1A-AS1. e, f The specific phosphatase inhibitor of SHP-1, PTP inhibitor III (PTP III), reverse the inhibitory effect of HNF1α (e) or HNF1A-AS1 (f) on the migration and invasion of Huh-7 cells. Data represent the mean ± SD of triplicate experiments. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. g A schematic model representing that HNF1α increases the activity of SHP-1 in human HCC cells via upregulating HNF1A-AS1

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