Fig. 5

ZEB1 transcriptionally suppresses miR-190. a The expression of miR-190 in MCF10A cells transfected with EMT-related transcription factors or control cells, as determined by RT-qPCR. b Promoter analysis of miR-190. Different putative miR-190 promoter region (left) were inserted in front of the pGL3-basic luciferase reporter and luciferase activity was measured. c Determination of the core promoter of miR-190. Two E-boxes were located on the core promoter of miR-190 (− 300 to + 1) and luciferase activity was measured. d miR-190 promoter activity was measured in MCF10A cells transfected with TWIST1, SNAI1, or ZEB1. e Mutation of E-box sequence prevents ZEB1 from inhibiting the miR-190 promoter. f Interaction between TWIST1, SNAI1, or ZEB1 and the miR-190 promoter sequence in MCF10A cells, as determined by ChIP assay. g Luciferase activity of miR-190 promoter in indicated cells. h The miR-190 expression in indicated cells as determined by RT-qPCR. ***P < 0.001, **P < 0.01