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Fig. 7 | Molecular Cancer

Fig. 7

From: SALL1 functions as a tumor suppressor in breast cancer by regulating cancer cell senescence and metastasis through the NuRD complex

Fig. 7

SALL1 over-expression in breast cancer cells inhibited tumor growth and development in vivo. a Over-expression of SALL1 in E0771 breast cancer cells dramatically inhibited tumor growth in NSG immunodeficient mice. However, SALL1 over-expression in B16F0 melanoma cells did not affect tumor development. E0771 (2 × 105/mouse) and B16F0 (1 × 105/mouse) tumor cells infected with lentivirus carrying SALL1, mSALL1 or vector, were subcutaneously injected into NSG mice. Tumor volumes were measured and presented as mean ± SD (n = 5 mice per group). P values were determined by the one-way analysis of variance (ANOVA). Similar results were obtained in three repeated experiments. b Representative image of the xenograft tumors obtained from the indicated groups at the endpoint of the experiments (day 21). c E0771 breast cancer cells over-expressed SALL1 had much lower tumor weight compared with that of other groups. Furthermore, SALL1 over-expression in B16F0 melanoma cells did not affect tumor weight. Results shown are mean ± SD of the xenograft tumor weights from the indicated groups in the two models at the endpoint of the experiments (day 21) (n = 5 mice per group). **p < 0.01, compared with the control groups transfected with mSALL1 and vector using unpaired t-test. d Large amounts of senescent tumor cells were observed in SALL1-transfected E0771 tumor tissues in NSG mice. SA-β-Gal expression was determined in the tumor frozen tissues from different groups at the endpoint of experiment. Left panels are photomicrographs of SA-β-Gal expression in tumor tissues from different groups. Right panel is the mean ± SD of SA-β-Gal+ cell numbers per high microscope field (× 400) in the tumor tissues from 5 mice of each group. **p < 0.01, compared with the control mice of mSALL1 and vector groups using unpaired t-test

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