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Fig. 8 | Molecular Cancer

Fig. 8

From: SALL1 functions as a tumor suppressor in breast cancer by regulating cancer cell senescence and metastasis through the NuRD complex

Fig. 8

SALL1 over-expression in breast cancer cells inhibited tumor metastasis in vivo. a Over-expression of SALL1 in E0771 breast cancer cells significantly inhibited the migration of tumor cells compared with the control mSALL1 and vector-transfected tumor cells in the wound closure assays. Data shown are from three independent experiments with similar results. b Over-expression of SALL1 in E0771 breast cancer cells markedly suppressed the tumor cell migration and metastasis in NSG mice. Lentivirus-transfected E0771 tumor cells were stained with VivoTag®680 XL and then injected tail intravenously (5 × 104/mouse) into NSG mice. Mice were imaged with the IVIS Spectrum at different time points following the tumor cell adoptive transfer into NSG mice. Data shown are the dorsal, ventral, and right lateral images of representative of 5 mice per group at 2 h and day 5. Color bar represents signal intensity scales over whole body. c and d Over-expression of SALL1 in E0771 cells dramatically decreased tumor macro-metastatic/colonized numbers in lung and liver surfaces. Representative images shown in (c) are a representative of mouse lungs and livers obtained from the indicated groups at the endpoint of the experiments. Tumor colonized spots were counted and results shown are mean ± SD in (d) (n = 5 mice per group). **p < 0.01, compared with the control groups transfected with mSALL1 and vector using unpaired t-test. e H & E staining on sections from embedded lung tissues showed that high amount of tumor cells infiltrated into lungs obtained from control groups (mSALL1 and vector), but not from the SALL1 transfection group

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