Skip to main content
Fig. 1 | Molecular Cancer

Fig. 1

From: Enhanced expression of histone chaperone APLF associate with breast cancer

Fig. 1

Enhanced APLF expression mark breast cancer. a Representative picture from tissue array (US biomax Inc., #OD-CT-RpBre03-004) for invasive ductal breast carcinoma (IDC) with 31 matched control and normal (adjacent tissue or ANT) sections were investigated for the expression of APLF by IHC following standard protocol. Scale bar: 50 μm. b Plots represent expression of APLF in adjacent normal tissues (ANT) and matched tumors respectively. Expression values were determined by histo-scoring and expressed as median with 95% confidence interval. Statistical significance was determined using Wilcoxon rank sum test. A.U. = Arbitrary Unit. c TCGA study on invasive breast carcinoma samples was analyzed. Pie chart represents the percentage of alteration in APLF mRNA expression among different subtypes of breast cancer patients [5]. d Different breast cancer cell lines were considered for the expression of APLF with varying degree of invasive potential. mRNA and protein were isolated from all these cell lines and observed for the expression of APLF by qRT-PCR (upper panel) and western blot analysis (lower panel). Error bar = S.E.M for three independent experiments. Statistical analysis was performed using Student t-Test function, *p < 0.05, **p < 0.01. Band intensity was measured by ImageJ software, RBI = Relative Band Intensity. A representative image for the blot has been presented

Back to article page