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Fig. 2 | Molecular Cancer

Fig. 2

From: Long noncoding RNA AFAP1-AS1 predicts a poor prognosis and regulates non–small cell lung cancer cell proliferation by epigenetically repressing p21 expression

Fig. 2

AFAP1-AS1 regulates NSCLC cell proliferation in vitro. a Analysis of AFAP1-AS1 expression levels in NSCLC cell lines compared with a normal bronchial epithelial cell line (16HBE) using qRT-PCR. b The relative expression levels of AFAP1-AS1 in A549 and SPCA1 cells transfected with si-NC or si-AFAP1-AS1 (si-AFAP1-AS1 1# and si-AFAP1-AS1 2#) were measured using qPCR. c MTT assays were performed after transfection to determine the cell viability of the transfected A549 and SPCA1 cells. d Colony-forming assays were conducted after transfection to determine the proliferation of the transfected A549 and SPCA1 cells. The colonies were counted and captured. e BrdU assays were used to detect the cell proliferation after transfection, respectively. f After transfection, the cell cycle stages of A549 and SPCA1 cells were analyzed by flow cytometry. The bar chart represents the percentages of cells in the G1/G0, S, or G2/M phases, as indicated.*, P < 0.05, and **, P < 0.01

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Molecular Cancer

ISSN: 1476-4598