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Fig. 6 | Molecular Cancer

Fig. 6

From: Long noncoding RNA AFAP1-AS1 predicts a poor prognosis and regulates non–small cell lung cancer cell proliferation by epigenetically repressing p21 expression

Fig. 6

AFAP1-AS1 epigenetically silences p21 transcription by binding to EZH2. a Western blot analysis of p21 protein level in A549 after AFAP1-AS1 knockdown. b QPCR and Western blot analysis of p21 mRNA and protein level in A549 after EZH2 knockdown. c ChIP-qPCR of EZH2/H3K27me3 of the promoter region of p21 genes locus after siRNA treatment targeting AFAP1-AS1 in A549 cells. Antibody enrichment was quantified relative to the amount of input DNA. Antibody directed against IgG was used as a negative control. d AFAP1-AS1 were negatively correlated with p21 expression in tissues. *, P < 0.05, and **, P < 0.01

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