Fig. 3

LncRNA-NEAT1 modulates ATGL expression in HCC cells and disrupts the lipolysis of hepatoma cells via ATGL in vitro. a Effects of inhibition of H19/ZFAS1/HULC/DANCR/SNHG20/NEAT1 expression by siRNA on ATGL expression as determined by real-time PCR analysis. b Real-time PCR analysis of NEAT1 expression in HCC cell lines. c Real-time PCR analysis showing the effect of sh-NEAT1 on ATGL expression in HCCLM3 and SK-Hep-1 cells (upper panel). Western blot analysis showing the effect of sh-NEAT1 on ATGL expression in HCCLM3 and SK-Hep-1 cells (lower panel). d and e Intracellular DAG and FFA levels determine the effect of sh-NEAT1 (or ATGL, sh-ATGL, Atglistatin) on lipolysis. f Real-time PCR analysis of NEAT1 expression in 40 pairs of HCC cancer tissues and matched para-cancer tissues. g Representative images of IHC detection of ATGL in HCC tissues with low NEAT1 expression (left panels) and high NEAT1 expression (right panels). h, i and j Correlation analyses revealed that relative mRNA levels of NEAT1 were positively associated with relative ATGL mRNA (or DAG, or FFA) levels in 40 HCC tissues. Pearson correlation coefficient was used to as a measure of association. Data are expressed as mean ± SD of three independent experiments. Statistical significance was concluded at *P < 0.05, **P < 0.01, ***P < 0.001; NS represents no statistical significance