Fig. 5

NEAT1-regulated abnormal lipolysis facilitates HCC cell growth in vivo. a Representative bioluminescence imaging of orthotopic HCCLM3 tumors. b Volume of HCCLM3 orthotopic tumors was determined at different timepoints. c The ratio of liver weight/body weight. d Representative images of immunohistochemical detection of Ki-67 and ATGL in orthotopic HCC tissues. e Representative bioluminescence imaging of HCCLM3 orthotopic HCC tumors in mice intraperitoneally injected with 20 mg/kg DAG+ FFA daily for 5 weeks. f Volume of HCCLM3 orthotopic tumors was determined at different timepoints. g The ratio of liver weight/body weight. h Representative images of immunohistochemical detection of Ki-67 in orthotopic HCC tissues. i and j Relative levels of DAG and FFA was determined in 100 mg orthotopic HCC tissues. Two-way ANOVA with the Bonferroni’s correction was used in different timepoints statistical analysis. Data are expressed as mean ± SD of three independent experiments. Statistical significance was concluded at *P < 0.05, **P < 0.01, ***P < 0.001