Fig. 7

Knockdown of NEAT1 attenuates HCC cell growth through miR-124-3p/ ATGL/ DAG+FFA/ PPARα signaling. a Real-time PCR analysis showing the effect of up-regulation of ATGL on PPARα expression in Huh7 and HepG2 cells. b Western blot analysis showing the effect of up-regulation of ATGL on PPARα expression in Huh7 and HepG2 cells. c Real-time PCR and western blot showing the effect of treatment with 16 μM and 32 μM DAG+FFA on PPARα expression in Huh7 and HCCLM3 cells. d Real-time PCR analysis and western blot analysis revealed that the sh-NEAT1 down-regulated PPARα expression in HCCLM3 and SK-Hep-1 cells, whereas miR-124-3p inhibitor treatment (or overexpression of ATGL/treatment with DAG+FFA) blocked this process. Data are expressed as mean ± SD of three independent experiments. Statistical significance was concluded at *P < 0.05, **P < 0.01, ***P < 0.001