Fig. 1

TIGAR expression is increased in high metastatic lung cancer cells, affecting cell migration, invasion and adhesion in vitro. (a-b) Wound healing assays and Trans-well analysis to determine the migration and invasion of A549, H1299, H1650, H1975 and PC9, respectively. (c-d) Statistical analysis was to quantify the migratory and invasive ability of five NSCLC cells. (e) Real-time PCR and Western blot analysis to determine endogenous TIGAR expression of five NSCLC cells. (f) Real-time PCR and Western blot analysis to respectively quantify mRNA and protein expression of TIGAR after transfection with siTIGAR (or siNC as control) for 48h. (g-h) Cells were transfected with siTIGAR (or siNC as control) for 48h were collected to determine the migration and invasion capability by wound-healing assays and Trans-well assay, respectively. (i-j) Statistical analysis was to quantify cells migratory and invasive ability upon TIGAR knockdown. (k) H1299 and A549 cells were infected with lentivirus with shTIGAR and Green fluorescent protein. To get more effective knockdown, monoclonal cell was to screen in A549. Monoclonal cells A549-shTIGAR#7 was the A549-shTIGAR in following assays. (l) Wound-healing assay for H1299 and A549 cells stably knockdown TIGAR or negative control. (m) Trans-well invasion assay for A549 cells stably knockdown TIGAR or negative control. (n) The effect of TIGAR on cell adhesion activity was assessed in A549