Fig. 5
From: Met is involved in TIGAR-regulated metastasis of non-small-cell lung cancer
TIGAR regulates the expression of Met protein. (a) Analysis of Western Blot showed the effect of TIGAR on Met in A549 and H1299 cells after being transfected with siTIGAR (or siNC as control) for 48h. (b) Western Blot analysis showed the effect of Met on TIGAR in NSCLC cells after being transfected with siMet (or siNC as control) for 48h. (c) The analysis of Western Blot showed the effect of TIGAR on Met in stably knockdown TIGAR cells. (d) The analysis of Western Blot showed the effect of Met on TIGAR in stably knockdown Met cells. (e) Overexpression TIGAR enhanced the protein levels of Met in two lines of NSCLC cells after being transfected with Flag-TIGAR (or empty vector as control) for 48h. (f) Knockdown TIGAR had not significantly affected on mRNA levels of Met in A549 and H1299 that transfected with siTIGAR (or siNC as control) for 48h. (g) Immunoprecipitation (IP) assays showed there was not directly interaction between TIGAR and Met