Fig. 4From: The long non-coding RNA PTTG3P promotes cell growth and metastasis via up-regulating PTTG1 and activating PI3K/AKT signaling in hepatocellular carcinomaLncRNAPTTG3P inhibits cellular apoptosis of HCC cells. (a) HepG2 and Hep3B cells with enhanced or silenced PTTG3P expression were stained with a combination of annexin V and 7-AAD and analyzed by FACS. Cells positive for annexin V staining were counted as apoptotic cells. (b) The HepG2 and Hep3B cells over-expressing PTTG3P were treated with 5-fluorouracil for 48 h, stained with a combination of annexin-V and 7-AAD, and analyzed by flow cytometry. (c) The bar graph showed the percentage of apoptotic cells. The results are means of 3 independent experiments ± SD from 3 independent experiments. *, P < 0.05Back to article page