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Fig. 8 | Molecular Cancer

Fig. 8

From: Long noncoding RNA HULC accelerates liver cancer by inhibiting PTEN via autophagy cooperation to miR15a

Fig. 8

HULC activates AKT-PI3K-mTOR pathway via PTEN reduction in liver cancer cells. a Western blotting with anti-PTEN, anti-pPTEN, anti-EGFR (Tyr845),anti-AKT,anti-pAKT,anti-TmTOR,anti-PI3K,anti-pPI3Kanti-mTOR,anti-pmTOR, anti-JUN, anti-Survivin and RT-PCR with HULC primer in Hep3B cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC,and pCMV6-A-GFP -HULC plus pCMV6-XL5-PTEN,respectively. b (left) CHIP with anti-JUN followed by PCR with H-Ras promoter primers in Hep3B cell line. IgG CHIP as negative control. H-Ras promoter as INPUT. (right) quantitative CHIP. c The assay of H-Ras promoter luciferase activity in Hep3B. d (left) The RT-PCR with H-Ras primers in Hep3B cell line. (right) The real-time RT-PCR. e (left) Western blotting with anti-H-Ras in Hep3B cell line. (right) The density analysis of band

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Molecular Cancer

ISSN: 1476-4598