Fig. 4

SNAI2 is a direct target of miR-25. a Sequence alignment showing the complementarity between miR-25 and the human SNAI2 gene. The matched base pairs in the seed region are in red and green. b Luciferase reporter activities of chimeric vectors carrying the luciferase gene and a fragment of the SNAI2 3′-UTR containing the wild-type or mutated miR-25 binding site. c Overexpression of miR-25 had no effect on the SNAI2 mRNA level. d Overexpression of miR-25 repressed the protein expression of SNAI2. e Knockdown of miR-25 increased SNAI2 protein expression. n = 5 independent experiments. **P < 0.01 vs. miR-Ctrl or NC