Fig. 6

Verification of candidate gene differential expression using RT-qPCR on recipient OK113 cells. a Each bar represents differential mean ± SEM gene expression (Log₂ Ratio) of 8 exosomes (NOK368, OK113, NK4, SqCC/Y1, CaLH2, Ca1, SVpgC2a, SVFN8)-transfected OK113 compared with untransfected OK113. b Each bar represents differential mean ± SEM gene expression (Log₂ Ratio) of 5 cancer exosomes (SqCC/Y1, CaLH2, Ca1, SVpgC2a, SVFN8)-transfected OK113 vs 3 normal exosomes (NOK368, OK113, NK4)-transfected OK113. Coloured bars (red for upregulated and green for downregulated genes) represent differential expression patterns correlated with results obtained by microarray experiments in Fig. 5. Grey bars represent insignificant and/or discordance expression patterns with results obtained by microarray. Statistical t-test *P < 0.05, **P < 0.01 and ***P < 0.001. c table listing all validated candidate genes with brief description of their putative functions. Further 8 genes were selected for exosome time and dose-response analysis on OK113 cells in Fig. 7