Fig. 3
From: MASTL induces Colon Cancer progression and Chemoresistance by promoting Wnt/β-catenin signaling
Cell are arrested in G2M phase as a result of MASTL knockdown in colon cancer. Control and MASTL knockdown cells were synchronized in serum free conditions for 72 h after which are treated with RO3306 for 16 h and then grown for one hour in fresh media. Cells were fixed immediately and cell cycle analysis was carried out via FACS. RO3306 is a selective ATP-competitive inhibitor of CDK1 that reversibly arrests proliferating human cells at the G2/M phase border, and the arrested cells enter mitosis rapidly after release from the G2 block [8] (a) HCT116 MASTL knockdown cells were unable to overcome the G2M block as compared to control cells (b) Similarly, the SW620 MASTL knockdown cells were also significantly arrested in G2M phase. For graphs, data represent mean ± SD; *, P < 0.01